Applications of human organoids in the personalized treatment for digestive diseasesArticle Published on 2022-09-272022-11-16 Journal: Signal Transduction and Targeted Therapy [Category] SARS, 유전자 메커니즘, [키워드] application approach build Chemotherapy Clinical treatment CRISPR-Cas9 digestive digestive system disease Diseases dose Drug screening epithelial facilitated Genetic helping highlight human physiology in vitro include Infection Intervention mechanism model system organ Organoid over pathogenic pathogenic mechanism Patient Perspective physiological Research SARS-CoV-2 targets test model therapy tissue Traditional Treatment [DOI] 10.1038/s41392-022-01194-6 PMC 바로가기
Smartphone-Based SARS-CoV-2 and Variants Detection System using Colorimetric DNAzyme Reaction Triggered by Loop-Mediated Isothermal Amplification (LAMP) with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)Article Published on 2022-07-262022-11-16 Journal: ACS Nano [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Accuracy acute respiratory syndrome affected blind Cas9 clinical samples clinical sensitivity coronavirus COVID-19 COVID-19 patient CRISPR CRISPR-Cas9 D614G detection diagnosed Diagnosis diagnostic disease Diseases eliminated expected False-positive isothermal amplification machine learning Mutation Omicron variant Palindromic palindromic repeat patients Protein raised reaction Repeat S gene SARS-CoV-2 SARS-CoV-2 delta sensitivity Smartphone specificity System T478K the SARS-CoV-2 triggered variant variants variants. [DOI] 10.1021/acsnano.2c04840 PMC 바로가기
ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1ESCPE-1은 SARS-CoV-2 숙주 인자 Neuropilin-1의 역행 엔도솜 분류를 매개합니다Article Published on 2022-06-212022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 bacterial pathogen cargo Cell culture cellular homeostasis characterized complex coronavirus COVID-19 CRISPR-Cas9 develop endosomal endosome exit facilitate Host identify Infection Lipid lipids maintain membrane methodology neuropilin Neuropilin-1 NRP1 Pathogens peptide Protein proteomic reduce SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus sorting nexin. subcellular subunit the SARS-CoV-2 transmembrane protein virus [DOI] 10.1073/pnas.2201980119 PMC 바로가기 [Article Type] Article
The State-of-the-Art of Gene Editing and its Application to Viral Infections and Diseases Including COVID-19최신 유전자 편집 기술과 코로나19를 포함한 바이러스 감염 및 질병에 적용Review Published on 2022-06-092022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Antiviral application Biotechnology Cas9 caused combating contribute coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pandemic CRISPR CRISPR-Cas9 CRISPR/Cas9 diagnostic disease Disease progression Diseases domain feature gene editing Gene therapy help highlight highly pathogenic implication Including Infectious diseases mechanism Multiple pandemic pandemic disease public health SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 shown therapeutic therapy Vaccines viral infection Viral infection. virus [DOI] 10.3389/fcimb.2022.869889 PMC 바로가기 [Article Type] Review
CRISPR-Cas9 Targeting of Hepatitis B Virus Covalently Closed Circular DNA Generates Transcriptionally Active Episomal VariantsArticle Published on 2022-04-262023-06-13 Journal: mBio [Category] B형 간염, Fulltext, [키워드] covalently closed circular DNA CRISPR-Cas9 Hepatitis B virus. [DOI] 10.1128/mbio.02888-21 PMC 바로가기
Tacrolimus-resistant SARS-CoV-2-specific T cell products to prevent and treat severe COVID-19 in immunosuppressed patientsResearch article Published on 2022-02-272022-10-05 Journal: Molecular Therapy [Category] 치료제, [키워드] acute respiratory syndrome adoptive immunotherapy analyses approach Autoimmunity bystander convalescent Coronavirus-2 Course COVID-19 COVID-19 cases COVID-19 immunopathology CRISPR-Cas9 death Donor Efficiency efficient clearance flow cytometry high risk immune response immunopathology Immunosuppressed immunosuppressed patient Immunosuppression Immunotherapy increased risk Infection manifested Patient Prevent receive recipient recipients reduced ribonucleoprotein RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-specific T cell Sequencing severe coronavirus disease severe COVID-19 single-cell single-cell level solid organ Solid organ transplant recipients SOT SOT patient specificity T cell T cell activation T cell receptor T cell therapy TCR therapeutic transfer treat Vaccine Vaccines viral clearance viral infection [DOI] 10.1016/j.omtm.2022.02.012 [Article Type] Research article
Recent Advances and Future Directions in the Understanding of Mucormycosis점막염에 대한 이해의 최근 발전과 향후 방향Review Published on 2022-02-242022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, 유전자 메커니즘, 진단, [키워드] acquired resistance advance analysis antifungal resistance caused characterization COVID-19 patient CRISPR CRISPR-Cas CRISPR-Cas9 Diagnosis direction effective expected Factor fungal fungi Future genetic manipulation genetic model genomic in some incidence Infection intractable involved knowledge macrophages molecular mechanism molecular mechanisms Mucorale Mucorales Mucormycosis Pathogenesis phagocytosis phagocytosis. recent reduced the disease Treatment understanding virulence virulence factors whole genome sequence [DOI] 10.3389/fcimb.2022.850581 PMC 바로가기 [Article Type] Review
The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entrySARS-COV-2 스파이크 (S)와 ZDHHC5/GOLGA7의 상호 작용 및 S 단백질의 세포 내 위치, 팔미 토일 화 및 슈도 바이러스 진입에 미치는 영향Research Published on 2021-12-272022-09-10 Journal: Virology Journal [Category] SARS, 치료기술, [키워드] A549 acute respiratory syndrome acute respiratory syndrome coronavirus Affect affected Affinity purification analyzed Antiviral APT2 assays Biotin Cas9 Cell cell line cell lines cell types cells co-immunoprecipitation co-IP Colocalization complex coronavirus CRISPR CRISPR-Cas9 cysteine cysteine residue determine domain Effect Efficiency endodomain enzymatic activity enzyme fluorescence microscopy GOLGA7 had no HEK293T HEK293T cell HEK293T cells Hela HeLa cells host protein host proteins independent independent of interact Interaction knowledge mass spectrometry no significant effect palmitoylation Protein provide pseudovirus entry reason Renilla Renilla luciferase respiratory Result S protein S proteins SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 S protein SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant effect significantly Spike protein subcellular localization the cell the S protein virus entry virus infection Virus-host interaction ZDHHC5 ZDHHC5/GOLGA7 [DOI] 10.1186/s12985-021-01722-w PMC 바로가기 [Article Type] Research
Recent Progress and Future Prospective in HBV Cure by CRISPR/CasReview Published on 2021-12-212023-06-02 Journal: Viruses [Category] B형 간염, [키워드] Chronic hepatitis B (CHB) CRISPR-Cas9 gene editing HBV cure Hepatitis B virus (HBV) [DOI] 10.3390/v14010004 PMC 바로가기 [Article Type] Review
Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell GrowthResearch Articles Published on 2021-12-012022-10-31 Journal: The CRISPR journal [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome antibody application applied approach autophagy Cell cell growth cell line cell lines Combination coronavirus CRISPR-Cas9 Donor Dynamics Effect Effectiveness fixation growth high-content imaging highlight identify inducer inhibitor lack methodology mitochondrial modified cell line ORF3a pathological change physiological Plasmid Protein role SARS-CoV-2 Seven staining Support Tagging target Viral protein [DOI] 10.1089/crispr.2021.0041 PMC 바로가기 [Article Type] Research Articles